R&D Systems™ Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 5-25 ng/mL in the presence of goat anti-mouse IgM.

Specifications

• Accession Number: O75888
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
• Gene ID (Entrez): 8741
• Molecular Weight (g/mol): M.W. (Observed): 26 kDa, reducing conditions; M.W. (theoretical): 21.7 kDa
• Name: APRIL/TNFSF13
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human APRIL/TNFSF13 protein HA (YPYDVPDYA) GCN4-IZ GGGSGGGSGGGS Human April (Ala105-Leu250) (N-terminus) Accession # O75888 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Noncovalently-linked homotrimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TNFSF13
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: APRIL/TNFSF13