R&D Systems™ Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

Catalog No.	Quantity
342CD200	200 μg

Catalog No. 342CD200 Supplier R&D Systems™ Supplier No. 342CD200

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1 ) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Specifications

• Accession Number: P10747
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 940
• Molecular Weight (g/mol): M.W. (Observed): 60-70 kDa, reducing conditions; M.W. (theoretical): 42.2 kDa (monomer)
• Name: CD28 Fc Chimera
• Quantity: 200 μg
• Source: Mouse myeloma cell line, NS0-derived CD28 protein Human/Cynomolgus Monkey/Rhesus Macaque CD28 (Asn19-Pro152) (N-terminus) Accession # P10747 DMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD28
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD28