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R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)

Catalog No. 6420CL025CF
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
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Catalog No. Formulation
6420CL025CF Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
6420CL025 Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
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Catalog No. 6420CL025CF Supplier R&D Systems™ Supplier No. 6420CL025/CF
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 20 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).

Specifications

Accession Number P29965
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Gene ID (Entrez) 959
Molecular Weight (g/mol) M.W. (Observed): 22-26 kDa, reducing condtions; M.W. (theoretical): 21.6 kDa
Name CD40 Ligand/TNFSF5
Quantity 25 μg
Source Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein Hemagglutinin tag YPYDVPDYA GCN4-IZ (Met1-Ile29) GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) (N-terminus) Accession # P29965 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.01 EU / 1 μg of the protein by the LAL method.
Gene Symbol CD40LG
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein CD40 Ligand/TNFSF5
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