R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 20 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).

Specifications

• Accession Number: P29965
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): M.W. (Observed): 22-26 kDa, reducing condtions; M.W. (theoretical): 21.6 kDa
• Name: CD40 Ligand/TNFSF5
• Quantity: 25 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein Hemagglutinin tag YPYDVPDYA GCN4-IZ (Met1-Ile29) GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) (N-terminus) Accession # P29965 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD40LG
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: CD40 Ligand/TNFSF5