R&D Systems™ Recombinant Human Chitotriosidase/CHIT1 Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave 4-Methylumbelliferyl-beta-D-N,N’,N"e;-triacetylchitotriose (4-MU-TACT). The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: NP_003456
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 1118
• Molecular Weight (g/mol): M.W. (Observed): 52 kDa, reducing conditions; M.W. (theoretical): 51 kDa
• Name: Chitotriosidase/CHIT1
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human Chitotriosidase/CHIT1 protein Ala22-Asn466, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CHIT1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Chitotriosidase/CHIT1