R&D Systems™ Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Catalog No.	Quantity
325CT200	200 μg

Catalog No. 325CT200 Supplier R&D Systems™ Supplier No. 325CT200

Description

New rh CTLA-4 Available! Increased activity; Enhanced purity; Lower endotoxin specification; CHO expressed!

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1 ) in the presence of PHA.

Specifications

• Accession Number: Q6GR94
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 1493
• Molecular Weight (g/mol): M.W. (theoretical): 40 kDa (monomer)
• Name: CTLA-4 Fc Chimera
• Quantity: 200 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CTLA-4 protein Human CTLA-4 (Ala37-Phe162) (N-terminus) Accession # Q6GR94 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag(C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CTLA4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CTLA-4