R&D Systems™ Human Granzyme B ELISpot Kit

Catalog No.	Quantity
XEL2906	1 Pack (6 Plates)

Catalog No. XEL2906 Supplier R&D Systems™ Supplier No. XEL2906

Description

For the quantitative determination of the frequency of cells releasing human Granzyme B

The Human Granzyme B ELISpot assay is designed for the detection of Granzyme B secreting cells at the individual single cell level and can be used to quantitate the frequency of human Granzyme B secreting cells. ELISpot assays are well suited for monitoring immune responses to various treatments and therapies and have been used for the quantitation of antigen-specific CD4 and/or CD8 T cells responses. Other methods for assessment of antigen-specific T cells responses, such as the chromium release assay with quantitation by limiting dilution, are tedious and require previous in vitro expansion of T cells for several days. These assays typically are not suitable for measuring infrequent T cell responses that occur at less than 1 in 1000. ELISpot assays are highly reproducible and sensitive and can be used to measure responses with frequencies well below 1 in 100,000. ELISpot assays do not require prior in vitro expansion of T cells and are suitable for high-throughput analysis using only small volumes of primary cells. As such, ELISpot assays are useful tools for research in vaccine development and for the monitoring of various clinical trials.

Specifications

• Antigen: Granzyme B
• Quantity: 1 Pack (6 Plates)
• Synonym: C11, CCPI, CGL1, CGL-1, CSPB, CSP-B, CTLA1, CTLA-1, CTSGL1, Fragmentin-2, granzyme B, Granzyme-2, GranzymeB, GRB, GrzB, GZMB, HLP, SECT
• Content And Storage: Store the unopened product at 2°C to 8°C. Do not use past expiration date.
• Gene ID (Entrez): 3002
• Gene Symbol: GZMB
• Host Species: Human