R&D Systems™ Recombinant Human His6-Ataxin-3 Protein, CF

Catalog No.	Quantity
E341050	50 μg

Catalog No. E341050 Supplier R&D Systems™ Supplier No. E341050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Recombinant Human His6-Ataxin-3 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Ataxin-3 concentration of 10-100 nM when using K63-linked tetra-Ubiquitin Rhodamine (UC-355) as a substrate. Higher enzyme concentrations may be required for other substrates.

Specifications

• Accession Number: AAH33711
• For Use With (Application): Enzyme Activity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Gene ID (Entrez): 4287
• Molecular Weight (g/mol): M.W. (theoretical): 44 kDa
• Name: His6 Ataxin-3
• Quantity: 50 μg
• Source: E. coli-derived human Ataxin-3 protein Contains an N-terminal Ser-Tyr-Tyr and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: ATXN3
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: Ataxin-3