R&D Systems™ Recombinant Human His6-ATG4B Protein, CF

Catalog No.	Quantity
E400025	25 μg

Catalog No. E400025 Supplier R&D Systems™ Supplier No. E400025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human His6-Apg4b/ATG4B is a Apg8-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg4b/ATG4B concentration of 10-100 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

Specifications

• Accession Number: Q9Y4P1
• For Use With (Application): Enzyme Activity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
• Gene ID (Entrez): 23192
• Molecular Weight (g/mol): M.W. (theoretical): 46 kDa
• Name: His6 ATG4B
• Quantity: 25 μg
• Source: E. coli-derived human ATG4B protein Contains an N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: ATG4B
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
• Protein: ATG4B