R&D Systems™ Recombinant Human IL-2 Protein

Description

Version with mutation found in aldesleukin Available!

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).

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Specifications

• Accession Number: P60568
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in acetonitrile and TFA with BSA as a carrier protein.
• Gene ID (Entrez): 3558
• Molecular Weight (g/mol): 13 kDa, under reducing conditions.
• Name: IL-2
• Quantity: 10 μg
• Source: E. coli-derived human IL-2 protein Ala21-Thr153, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: IL2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store (at 2°C to 8°C.)
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: IL-2