R&D Systems™ Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-7 R alpha/CD127 Fc Chimera (0.25 μg/mL, 100 μL/well) is immobilized on a goat anti-human IgG Fc antibody (Catalog # G-102-C ) coated plate, the concentration of recombinant human IL-7 that produces 50% of the optimal binding response is found to be 7.5-60 ng/mL.

Specifications

• Accession Number: BAG62793.1
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS.
• Gene ID (Entrez): 3575
• Molecular Weight (g/mol): 54.5 kDa (monomer)
• Name: IL-7R alpha/CD127
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived human IL-7 R alpha/CD127 protein, (N-terminus) Human IL-7 R alpha (Glu21-Lys261) Accession &Num; BAG62793.1 IIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Gene Alias: CD127, CD127 antigen, CD127ILRA, CDW127, IL-7 R alpha, IL-7 receptor subunit alpha, IL7R, IL7R alpha, IL-7R subunit alpha, IL7RA, IL-7RA, IL-7R-alpha, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin-7 receptor subunit alpha
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.