R&D Systems™ Recombinant Human ITCH/AIP4 Isoform 2 Protein, CF

Catalog No.	Quantity
E3260100	100 μg

Catalog No. E3260100 Supplier R&D Systems™ Supplier No. E3260100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Recombinant Human ITCH/AIP4 is a HECT domain Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ITCH/AIP4 concentration of 0.1-0.5 μM.

Specifications

• Accession Number: Q96J02-2
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 2 mM TCEP
• Gene ID (Entrez): 83737
• Molecular Weight (g/mol): M.W. (theoretical): 99 kDa
• Name: ITCH/AIP4 Isoform 2
• Quantity: 100 μg
• Source: E. coli-derived human ITCH/AIP4 protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: ITCH
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
• Protein: ITCH/AIP4