R&D Systems™ Recombinant Human LIF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 ng/mL.

Specifications

• Accession Number: P15018
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 3976
• Molecular Weight (g/mol): M.W. (Observed): 19 kDa, reducing conditions; M.W. (theoretical): 19.6 kDa
• Name: LIF
• Quantity: 25 μg
• Source: E. coli-derived human LIF protein Pro24-Phe202
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor), Melanoma-derived LPL inhibitor, MLPLI
• Gene Symbol: LIF
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: LIF