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R&D Systems™ Recombinant Human LIF Protein

Catalog No. 7734LF025CF
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Quantity:
100 μg
25 μg
500 μg
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
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Catalog No. Quantity Formulation
7734LF025CF 25 μg Lyophilized from a 0.2 μm filtered solution in PBS.
7734LF025 25 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
7734LF100CF 100 μg Lyophilized from a 0.2 μm filtered solution in PBS.
7734LF100 100 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
77-34L-F500 500 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
77-34L-F500CF 500 μg Lyophilized from a 0.2 μm filtered solution in PBS.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 ng/mL.

Specifications

Accession Number P15018
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 3976
Molecular Weight (g/mol) M.W. (Observed): 19 kDa, reducing conditions; M.W. (theoretical): 19.6 kDa
Name LIF
Quantity 25 μg
Source E. coli-derived human LIF protein Pro24-Phe202
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.01 EU / 1 μg of the protein by the LAL method.
Gene Alias CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor), Melanoma-derived LPL inhibitor, MLPLI
Gene Symbol LIF
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein LIF
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