R&D Systems™ Recombinant Human/Mouse/Rat Activin A Protein

Description

CHO derived

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.

Specifications

• Accession Number: P08476
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 3624
• Molecular Weight (g/mol): M.W. (Observed): 14 kDa, reducing conditions 24 kDa, non-reducing conditions; M.W. (theoretical): 13 kDa (monomer)
• Name: Activin A
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived Activin A protein Gly311-Ser426
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: INHBA
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: Activin A