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R&D Systems™ Recombinant Human MSP/MST1 Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Manufacturer:  R&D Systems™ 352MS010

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Catalog No. 352MS010


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Description

Description

Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by DuoSet IC human phospho-MSP R/Ron kit (Catalog # DYC1947 ). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 μg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.
Specifications

Specifications

MSP/MST1
Bioactivity
4485
MSP/MST1
10 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
<1.0 EU / 1 µg of the protein by the LAL method.
Unconjugated
Reconstitute at 100 µg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
AAA59872
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
M.W. (Observed): 81 kDa, 62 kDa and 30 kDa, reducing conditions; M.W. (theoretical): 78.5 kDa (MSP alpha/beta); 53.3 kDa (alpha chain), 25.2 kDa (beta chain)
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Mouse myeloma cell line, NS0-derived human MSP/MST1 protein Gln19-Gly711
Disulfide-linked heterodimer
MST1
Yes
SDS
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