R&D Systems™ Recombinant Human MSP/MST1 (CHO-expressed) Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet™ IC Kit (Catalog # DYC1947 ). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog # 1947-MS) is represent at 100 ng/mL (100 μg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.

Specifications

• Accession Number: AAA59872
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 4485
• Molecular Weight (g/mol): M.W. (Observed): 85 kDa, non-reducing conditions; M.W. (theoretical): 53 kDa (alpha-chain), 25 kDa (beta-chain)
• Name: MSP/MST1
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human MSP/MST1 protein Gln19-Arg483 (alpha-chain) & Val484-Gly711 (beta-chain)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked heterodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: MST1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: MSP/MST1