R&D Systems™ Recombinant Human MSP/MST1 (CHO-expressed) Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.
Manufacturer: R&D Systems™ 352MCH010
DescriptionMeasured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet™ IC Kit (Catalog # DYC1947 ). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog # 1947-MS) is represent at 100 ng/mL (100 μg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|<0.10 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 100 µg/mL in sterile PBS.|
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 85 kDa, non-reducing conditions; M.W. (theoretical): 53 kDa (alpha-chain), 25 kDa (beta-chain)|
|>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Chinese Hamster Ovary cell line, CHO-derived human MSP/MST1 protein Gln19-Arg483 (alpha-chain) & Val484-Gly711 (beta-chain)|