R&D Systems™ Recombinant Human Siglec-3/CD33 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 1-4 μg/mL.

Specifications

• Accession Number: AAA51948
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 945
• Molecular Weight (g/mol): M.W. (Observed): 67-85 kDa, reducing conditions; M.W. (theoretical): 53.4 kDa (monomer)
• Name: Siglec-3/CD33 Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Siglec-3/CD33 protein Human Siglec-3 (Asp18-His259) (Val257Leu) (N-terminus) Accession # AAA51948 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD33
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Siglec-3/CD33