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R&D Systems™ Recombinant Human Syndecan-1 Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Supplier: R&D Systems™ 2780SD050
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DescriptionMeasured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin (0.5 μg/mL, Catalog # 4305-FNB ). The ED50 for this effect is 2.5-10 μg/mL.
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 60-90 kDa, reducing conditions; M.W. (theoretical): 24.9 kDa|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.|
|Mouse myeloma cell line, NS0-derived human Syndecan-1/CD138 protein Gln18-Glu251, with a C-terminal 6-His tag|
|<0.10 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 500 µg/mL in PBS.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
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