R&D Systems™ Recombinant Human Thrombospondin-1 Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 2 x 104 cells/well are added to DTT treated THBS-1 coated plates (10 μg/mL, 100 μL/well), > 50% will adhere after 1 hour at 37 °C.

Specifications

• Accession Number: P07996-1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.
• Gene ID (Entrez): 7057
• Molecular Weight (g/mol): M.W. (Observed): 135 - 165 kDa, reducing conditions; M.W. (theoretical): 129 kDa
• Name: Thrombospondin-1
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Thrombospondin-1 protein Asn19-Pro1170, with Thr523Ala substitution and a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: THBS1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Thrombospondin-1