R&D Systems™ Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

Catalog No. ES002

Supplier: R&D Systems™ ES002

Description

Applications: Bioactivity, Binding Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).

It is also an excellent substrate for Trypsin, HGF activator and factor Xa.

Specifications

• Form: Liquid
• For Use With (Application): Bioactivity, Binding Activity
• Quantity: 1 mg
• Format: Supplied as a stock solution in dimethyl sulfoxide (DMSO) at a concentration of 10.0mg/mL or 4.3 mM.
• Molecular Weight (g/mol): Predicted MW: 1674 Da
• Product Type: Purity: >95%, by HPLC, Source: Synthetic
• Storage Requirements: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.