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R&D Systems™ Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

Catalog No. 12464L025CF
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
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Catalog No. Formulation
12464L025CF Lyophilized from a 0.2 μm filtered solution in PBS.
12464L025 Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 μg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ). Measured by its ability to bind Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B ) in a functional ELISA.

Specifications

Accession Number P41274
For Use With (Application) Bioactivity, Binding Activity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 8744
Molecular Weight (g/mol) M.W. (Observed): 41-44 kDa, reducing conditions; M.W. (theoretical): 25.7 kDa
Quantity 25 μg
Source Mouse myeloma cell line, NS0-derived mouse 4-1 BB Ligand/TNFSF9 protein Met 10-His tag GGGSGGGSGGGSIEGR Mouse 4-1 BB Ligand (Arg104-Glu309) (N-terminus) Accession # P41274 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Symbol Tnfsf9
Product Type 4-1BB Ligand/TNFSF9
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein 4-1 BB Ligand/TNFSF9
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