R&D Systems™ Recombinant Mouse CTLA-4 Fc Chimera Protein

Catalog No.	Formulation
434CT200	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
434CT200CF	Lyophilized from a 0.2 μm filtered solution in PBS.

Catalog No. 434CT200 Supplier R&D Systems™ Supplier No. 434CT200

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1 ) in the presence of PHA.

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Specifications

• Accession Number: Q6GTR6
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 1493
• Molecular Weight (g/mol): M.W. (Observed): 55 kDa, reducing conditions; M.W. (theoretical): 41 kDa (monomer)
• Name: CTLA-4 Fc Chimera
• Quantity: 200 μg
• Source: Mouse myeloma cell line, NS0-derived mouse CTLA-4 protein Mouse CTLA-4 (Ala37-Phe162) (N-terminus) Accession #Q6GTR6 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Ctla4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: CTLA-4