R&D Systems™ Recombinant Mouse Matriptase/ST14 Catalytic Domain

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014 ). The specific activity is >4,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: NP_035306
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and Glycerol.
• Gene ID (Entrez): 6768
• Molecular Weight (g/mol): M.W. (Observed): 27 kDa (major) and minor auto-activation fragments, reducing conditions; M.W. (theoretical): 3 kDa & 26 kDa
• Name: Matriptase/ST14 Catalytic Domain
• Quantity: 10 μg
• Source: E. coli-derived mouse Matriptase/ST14 protein Gly596-Val855, with an N-terminal Met and 6-His tag The protein was auto-activated and further purified.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: St14
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Matriptase/ST14