R&D Systems™ Recombinant Mouse PDGF R beta Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 6-48 ng/mL in the presence of 4 ng/mL recombinant human PDGF-BB.

Specifications

• Accession Number: P05622
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 5159
• Molecular Weight (g/mol): M.W. (Observed): 124 kDa, reducing conditions; M.W. (theoretical): 82.6 kDa (monomer)
• Name: PDGF R beta Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived mouse PDGF R beta protein Mouse PDGF R beta (Leu32-Lys530) (N-terminus) Accession # P05622 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Pdgfrb
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: PDGF R beta