R&D Systems™ Recombinant Mouse PDGF R beta Fc Chimera Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Supplier: R&D Systems™ 1042PR100
DescriptionMeasured by its ability to inhibit the biological activity of PDGF-BB using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 6-48 ng/mL in the presence of 4 ng/mL recombinant human PDGF-BB.
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 124 kDa, reducing conditions; M.W. (theoretical): 82.6 kDa (monomer)|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|<0.10 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 100 µg/mL in sterile PBS.|
|PDGF R beta|
|PDGF R beta Fc Chimera|
|Mouse myeloma cell line, NS0-derived mouse PDGF R beta protein Mouse PDGF R beta (Leu32-Lys530) (N-terminus) Accession # P05622 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)|
|>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
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