R&D Systems™ Recombinant Mouse TRH-degrading Ectoenzyme/TRHDE Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 2985ZN010

Catalog No. 2985ZN010

Description

Measured by its ability to cleave the pyroGlu-His bond in a fluorogenic peptide substrate, pyroGlu-His-Pro-7-amido-4-methylcoumarin (pEHP-AMC). The resulting HP-AMC was cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE ). The specific activity is >130 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q8K093
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Molecular Weight (g/mol): M.W. (Observed): 131 kDa, reducing conditions; M.W. (theoretical): 112 kDa
• Quantity: 10 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Gene Symbol: Trhde
• Recombinant: Recombinant
• Protein: TRH-degrading Ectoenzyme/TRHDE

• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 29953
• Name: TRH-degrading Ectoenzyme/TRHDE
• Source: Mouse myeloma cell line, NS0-derived mouse TRH-degrading Ectoenzyme/TRHDE protein Arg64-His1025, with an N-terminal 6-His tag
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.