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R&D Systems™ Recombinant Rat GDNF Protein

Catalog No. 512GF050CF
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Quantity:
10 μg
50 μg
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
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Catalog No. Quantity Formulation
512GF050CF 50 μg Lyophilized from a 0.2 μm filtered solution in PBS.
512GF010 10 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
512GF050 50 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
512GF010CF 10 μg Lyophilized from a 0.2 μm filtered solution in PBS.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ) at 1 μg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.

Specifications

Accession Number Q07731
For Use With (Application) Bioactivity, Binding Activity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 2668
Molecular Weight (g/mol) M.W. (theoretical): 15 kDa (monomer)
Name GDNF
Quantity 50 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein Ser78-Ile211
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <0.01 EU / 1 μg of the protein by the LAL method.
Gene Symbol Gdnf
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein GDNF
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