R&D Systems™ Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer

Description

Purified from E. coli containing a recombinant plasmid harboring the Methanobacterium thermoautotrophicum TDG gene

TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum. The optimal temperature for the enzyme is 65°C. The enzyme lacks significant AP lyase or endonuclease activity.

• One Unit is the amount of enzyme required to cleave 1pmole of an oligonucleotide duplex containing a T/G mismatch in 1 hour at 65°C
• Only the strand containing the T is cleaved
• TDG works effectively in heteroduplex analysis to detect C to T transitions

Specifications

• Content And Storage: Store at -20° C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80° C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 forimmediate use. TDG protein in storage buffer can survive for up to 24 hours at 37° C with less than 10% loss in activity.
• Quantity: 500 U
• Product Type: Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer