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R&D Systems™ Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer

Catalog No. 4070500EB
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500 U
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Catalog No. Quantity
4070500EB 500 U
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Catalog No. 4070500EB Supplier R&D Systems™ Supplier No. 4070500EB
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Description

Purified from E. coli containing a recombinant plasmid harboring the Methanobacterium thermoautotrophicum TDG gene

TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum. The optimal temperature for the enzyme is 65°C. The enzyme lacks significant AP lyase or endonuclease activity.

  • One Unit is the amount of enzyme required to cleave 1pmole of an oligonucleotide duplex containing a T/G mismatch in 1 hour at 65°C
  • Only the strand containing the T is cleaved
  • TDG works effectively in heteroduplex analysis to detect C to T transitions

Specifications

Content And Storage Store at -20° C in a manual defrost freezer. For long term storage, freeze in working aliquots at -80° C. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 4 forimmediate use. TDG protein in storage buffer can survive for up to 24 hours at 37° C with less than 10% loss in activity.
Quantity 500 U
Product Type Thermostable TDG Protein (Thymine DNA Glycosylase)/Buffer
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