R&D Systems™ Recombinant Yeast SUMO E1 (SAE1/UBA2) Protein, CF

Catalog No.	Quantity
E311025	25 μg

Catalog No. E311025 Supplier R&D Systems™ Supplier No. E311025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

S. cerevisiae SUMO E1 (SAE1/UBA2) is a member of the SUMO-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a SUMO-conjugating (E2) enzyme to conjugate SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial S. cerevisiae SUMO E1 (SAE1/UBA2) concentration of 50-500 nM.

Specifications

• Accession Number: Qo6624, P52488
• For Use With (Application): Enzyme Activity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1 mM DTT
• Gene ID (Entrez): 10055
• Molecular Weight (g/mol): M.W. (theoretical): 39 kDa (SAE1) 72 kDa (UBA2)
• Name: SUMO Activating Enzyme E1 (SAE1/UBA2)
• Quantity: 25 μg
• Source: E. coli-derived yeast SUMO Activating Enzyme E1 (SAE1/UBA2) protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: AOS1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
• Protein: SUMO Activating Enzyme E1 (SAE1)