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R&D Systems™ Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Catalog No. 211TBB010
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Quantity:
10 μg
50 μg
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
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Catalog No. Quantity Formulation
21-1TB-B010 10 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
21-1TB-B050 50 μg Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
21-1TB-B010CF 10 μg Lyophilized from a 0.2 μm filtered solution in PBS.
21-1TB-B050CF 50 μg Lyophilized from a 0.2 μm filtered solution in PBS.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.

Order Info

The product is shipped at ambient temperature. Upon receipt, store it immediately

Specifications

Accession Number P01374
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Gene ID (Entrez) 4049
Molecular Weight (g/mol) M.W. (Observed): 17-19 kDa, reducing conditions; M.W. (theoretical): 19 kDa
Name Lymphotoxin-alpha/TNF-beta
Preparation Method Reconstitute at 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Quantity 10 μg
Source E. coli-derived human Lymphotoxin-alpha/TNF-beta protein Leu35-Leu205, with an N-terminal Met
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.01 EU / 1 μg of the protein by the LAL method.
Gene Symbol LTA
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
Form Powder
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Lymphotoxin-alpha/TNF-beta
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