Invitrogen™ RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein

RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein

Manufacturer:  Invitrogen™ PV3805

Catalog No. PV3805

Description

RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.

Specifications

• Accession Number: NP_002871, Y340D, Y341D
• Molecular Weight: 66.4 kDa
• Gene Alias: RAF1 (cRAF) Y340D Y341D
• Protein Length: aa 306-648; Y340D, Y341D
• Protein Tag: GST Tag
• Assay Entry: Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
• Shipping Condition: Dry Ice
• Target Entry: RAF1 (cRAF) Y340D Y341D
• Expression System: Baculovirus
• Protein Form: Recombinant, Catalytic, Mutant
• Protein Subtype: Serine/Threonine Kinases
• Species: Human

• Gene ID (Entrez): 4773, 5894
• Quantity: 10μg
• KinaseFamily: RAF Kinase Family
• Recombinant: Yes
• Content And Storage: Storage Buffer: 50mM Tris, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, 50% glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Kinase Group: Ser⁄Thr Kinases: TKL Group
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate
• Target Gene: v-raf-1 murine leukemia viral oncogene homolog 1
• Protein Family: Kinases & Inhibitors
• Form: Liquid
• Validated Application: Kinase Assay
• Research Category: Signal Transduction

For Research Use Only. Not for use in diagnostic procedures.