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Invitrogen™ Rat RAGE (AGER) ELISA Kit

Catalog No. 502545404
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Quantity:
5 x 96 Tests
96 Tests

ELISA

The Rat RAGE (AGER) ELISA quantitates Rt RAGE in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt RAGE. Principle of the method The Rat RAGE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q63495
Assay Range 0.41 to 300 ng/mL
Assay Sensitivity 300 pg/mL
Calibration Range 300 pg/mL to 300 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Sufficient For 5 x 96 assays
Target Species Rat
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 81722
Gene Symbol Ager
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Development
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RAGE
Target RAGE
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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