Invitrogen™ RARA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1810A

Catalog No. PA1810A

Description

PA1-810A detects retinoic acid receptor (RAR) from human and mouse tissues and cells. This antibody does not detect RAR beta or gamma forms. PA1-810A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~50-58 kDa protein representing RAR alpha from Jurkat, CEM-C7, and IM9 cells. The PA1-810A immunogen is a synthetic peptide corresponding to residues C S(444) P S L S P S S H R S S P A T Q S P (462) of mouse RAR alpha. PA1-810A immunizing peptide (Cat. # PEP-004) is available for use in neutralization and control experiments.

RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

Specifications

• Antigen: RARA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RARA
• Gene Alias: Nr1b1; nuclear receptor subfamily 1 group B member 1; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RAR; RAR Alpha; RAR alpha 1; RARA; RAR-alpha; RARalpha1; retinoic acid; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; retinoic acid receptor; retinoic acid receptor alpha; retinoic acid receptor, alpha; retinoic acid receptor, alpha polypeptide; RRA
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Concentration: Conc. Not Determined
• Formulation: Whole serum, PBS with 0.05% sodium azide
• Gene Accession No.: P10276, P11416
• Gene Symbols: RARA
• Immunogen: Synthetic peptide corresponding to residues C S(444) P S L S P S S H R S S P A T Q S P (462) of mouse RAR alpha.
• Regulatory Status: RUO
• Gene ID (Entrez): 19401, 5914
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

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