Invitrogen™ RASA1 Monoclonal Antibody (B4F8)

Catalog No.	Quantity
MA4001	100 μg

Catalog No. MA4001 Supplier Invitrogen™ Supplier No. MA4001

Description

Mouse Monoclonal Antibody

MA4-001 detects GTPase activating protein (GAP) from human, mouse, rat, bovine and non-human primate tissues. MA4-001 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. Immunofluorescence staining of GAP in mouse fibroblast cells with MA4-001 results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. MA4-001 immunoprecipitates GAP that is complexed with the GAP related p190. The MA4-001 antigen is full length recombinant human GAP. Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 & SH3. Reconstitute with PBS.

TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 4,8, and 10. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner.

Specifications

• Antigen: RASA1
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: B4F8
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: RASA1
• Gene Accession No.: P09851, P20936, P50904
• Gene Alias: CMAVM; CM-AVM; Gap; GAPX; GTPase-activating protein; p120; p120 RAS GTPase activating protein; p120GAP; p120RASGAP; p120-rasGAP; PKWS; Ras GTPase-activating protein 1; ras GTPase-activating protein 1-like protein; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein RAS p21); RAS p21 protein activator (GTPase activating protein) 1; RAS p21 protein activator 1; Rasa; RASA1; RASGAP; triphosphatase-activating protein; unnamed protein product
• Gene Symbols: RASA1
• Host Species: Mouse
• Immunogen: Full length recombinant human GAP.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 218397, 25676, 282032, 5921
• Target Species: Human, Mouse, Rat, Monkey, Bovine
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a