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R&D Systems™ Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Catalog No. 91884L025CF
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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Catalog No. Formulation
91-884-L025CF Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
91-884-L025 Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
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Catalog No. 91-884-L025CF Supplier R&D Systems™ Supplier No. 91884L025/CF
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Rat 4-1BB/TNFRSF9 Fc Chimera (Catalog # 7968-4B ) is Immobilized at 100 ng/mL (100 μL/well), the concentration of Recombinant Rat 4-1BB Ligand/TNFSF9 that produces 50% optimal binding response is approximately 1-6 ng/mL. Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 5-30 ng/mL

Order Info

Ships at ambient temperature.

Specifications

Accession Number Q80WE6
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 8744
Molecular Weight (g/mol) M.W. (Observed): 33-43 kDa, reducing conditions; M.W. (theoretical): 24 kDa
Quantity 25 μg
Source Mouse myeloma cell line, NS0-derived rat 4-1 BB Ligand/TNFSF9 protein Arg103-Gly308, with an N-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Regulatory Status RUO
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Biological Activity 5 to 30ng/mL
Product Type Recombinant protein
Conjugate Unconjugated
Species Rat
Recombinant Recombinant
Reconstitution Reconstitute at 250 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein 4-1 BB Ligand/TNFSF9
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