CD49e Rat anti-Mouse, Brilliant Violet 421, Clone: 5H10-27 (MFR5), BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740017

Catalog No. BDB740017

Description

The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: CD49e
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Itga5; ITA5; Integrin alpha-5; Fibronectin receptor alpha; Fnra; VLA5 alpha
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 5H10-27 (MFR5)
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse Mast Cell Line MC/9
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only