Rat heart tissue lysate, Denatured; Abnova

Catalog No.	Quantity
89-014-502	200 μg

Catalog No. 89-014-502 Supplier Abnova Corporation Supplier No. L036W1

Description

Western Blotting

Specifications

• Tissue: Heart
• For Use With (Application): Western Blot
• Description: Whole tissue lysate (denatured)
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Preparation Method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Quantity: 200 μg
• Storage Buffer: In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
• Host Species: Rat
• Concentration: 3 mg/mL
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.