Rat heart tissue lysate, Non-denatured; Abnova

Supplier:  Abnova Corporation L036W2

Catalog No. 89-014-503

Description

Western Blotting, Immunoprecipitation

Specifications

• For Use With (Application): Immunoprecipitation, Western Blot
• Description: Whole tissue lysate (non-denatured)
• Preparation Method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
• Quantity: 200 μg
• Host Species: Rat
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

• Tissue: Heart
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Concentration: 5 mg/mL