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Invitrogen™ Rat IgG1 kappa Isotype Control (eBRG1), eBioscience™, Invitrogen™
Rat Isotype Control
Supplier: Invitrogen™ 14430182
Description
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA. Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
Specifications
| Rat IgG1 kappa | |
| Monoclonal | |
| 0.5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Rat | |
| 100 μg | |
| Isotype Control (Primary) | |
| 4°C | |
| Liquid |
| Control, Flow Cytometry, Immunohistochemistry (Frozen), Immunocytochemistry, Immunohistochemistry | |
| eBRG1 | |
| Unconjugated | |
| Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1 | |
| Affinity chromatography | |
| RUO | |
| Not Applicable | |
| Isotype Control | |
| IgG1 κ |
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Product Title
For Research Use Only.
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