Invitrogen™ Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Catalog No.	Quantity
50-112-8687	25 μg

Catalog No. 50-112-8687 Supplier Invitrogen™ Supplier No. 53403180

Description

Rat Monoclonal Isotype Control

The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: Alexa Fluor 488 Rat IgG2b Isotype Control has been reported for use in immunohistochemistry, immunocytochemistry, and flow cytometric analysis. Applications Tested: Alexa Fluor 488 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Please use at the same concentration as the experimental antibody. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.

Specifications

• Antigen: Rat IgG2b kappa
• Applications: Control, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry
• Classification: Monoclonal
• Clone: eB149/10H5
• Concentration: 0.5 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG2b κ

Frequently Asked Questions (FAQs)

Is an isotype control a must for flow cytometry analysis?

No. Many users are using unstained cells in combination with FMO controls to identify their positive populations.

What if I can't find the right isotype control for flow cytomtery?

The flow cytometry field is moving away from using isotype controls as they are not necessarily the most appropriate way to control for non-specific binding. Instead, they are using unstained cells to define the negative population, single stained controls to set compensation, and flow minus one (FMO) controls to set regions and gates.

You may want to consider whether using an isotype control is something you need to do. Here are some references you might want to look at:
-O'Gorman MR, Thomas J. (1999) Isotype controls-time to let go? Cytometry 38:78-80.

-Keeney M, Gratama JW, Chin-Yee IH et al. (1998) Isotype controls in the analysis of lymphocytes and CD34+ stem/progenitor cells by flow cytometry- time to let go! Cytometry 34:280-283.

-Hulspas R, O'Gorman MR, Wood BL et al. (2009) Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 76:355–364.

-Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H42-A2 Volume 27 No.16, 2007.

-Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H43-A2 Volume 27 No. 11, 2007.

If you do wish to use isotype controls, we offer a wide variety of species and fluorophores, search our Web site under "isotype antibody control."

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov