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Invitrogen™ Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet™ 805, eBioscience™

Catalog No. 368403181
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36-840-3181 50 μg
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Catalog No. 36-840-3181 Supplier Invitrogen™ Supplier No. 368403181
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Description

Rat Monoclonal Isotype Control

The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin. Applications Tested This eB149/10H5 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Our internal testing suggests that Brilliant Ul...

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Rat IgG2b kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eB149/10H5
Concentration 0.2 mg/mL
Conjugate Brilliant Ultraviolet 805
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG2b κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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