Gibco™ Rat IL-21 Recombinant Protein, PeproTech®

Catalog No.	Quantity
400-41-1MG	1 mg
40041100UG	100 μg
400-41-10UG	10 μg
40041250UG	250 μg
400-41-2UG	2 μg
40041500UG	500 μg

Catalog No. 400-41-1MG Supplier Gibco™ Supplier No. 400411MG

Description

Recombinant Protein

Recombinant Rat IL-21 is a 15.3 kDa protein containing 130 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Accession Number: A3QPB9
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 365769
• Molecular Weight (g/mol): 15.3 kDa
• Name: Rat IL-21
• Quantity: 1 mg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CVID11; C x 2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Common Name: IL-21
• Gene Symbol: IL21
• Biological Activity: Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MHKSSPQRPD HLLIRLRHLM DIVEQLKIYE NDLDPELLTA PQDVKGQCEH EAFACFQKAK LKPSNTGNNK TFINDLLAQL RRRLPAKRTG NKQRHMAKCP SCDLYEKKTP KEFLERLKWL LQKMIHQHLS
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.