Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Rat liver tissue lysate, Denatured; Abnova
Click to view available options
Quantity:
200 μg
Description
- Tissue: Liver
- Host: Rat
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In ready-to-use 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Western Blotting
Specifications
Specifications
For Use With (Application) | Western Blot |
Tissue | Liver |
Description | Whole tissue lysate (denatured) |
Lysis Buffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Preparation Method | Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
Quality Control Testing | 12.5% SDS-PAGE Stained with Coomassie Blue. |
Quantity | 200 μg |
Storage Buffer | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
Host Species | Rat |
Concentration | 3 mg/mL |
Show More |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction