Invitrogen™ Human RBP4 ELISA Kit

Catalog No.	Quantity
BMS2199	96 Tests

Catalog No. BMS2199 Supplier Invitrogen™ Supplier No. BMS2199

Description

ELISA

The Human Retinol-binding protein 4 (HuRBP-4) ELISA quantitates Hu RBP-4 in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu RBP-4. Principle of the method The Human RBP-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This protein belongs to the lipocalin family and is the specific carrier for retinol in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells.

Specifications

• Accession Number: P02753
• Assay Range: 0.063 to 4 ng/mL
• Assay Sensitivity: 0.034 ng/ml
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5950
• Gene Symbol: MCOPCB10,RDCCAS
• Interassay CV: 8.1%
• Intraassay CV: 5
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Target: RBP4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov