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Invitrogen™ ReadyProbes™ Reagent F-Actin Phalloidin Conjugates
Description
Includes
2 x 2.5 mL dropper bottles
ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle.
These ready-to-use solutions combines superior brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.
Features
- High-affinity staining of F-actin with superior specificity compared to antibody methods
- ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
- ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
- Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired
ReadyProbes format advantages
- Ready-to-use formulation—no need to dilute, weigh, or pipette
- Just use two drops per mL of medium to stain, incubate 30 minutes, wash, then image
- Stable at room temperature—keep handy on the benchtop
Applications
- For fixed and permeabilized cells or tissue
- Fluorescence microscopy (IF/IHC/ICC)
- High content screening, high content imaging
- Flow cytometry
Suggestions for use
- Both ActinRed 555 and ActinGreen 488 reagents may be added directly to fixed cells in full medium or buffer solutions
- In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Specifications
Specifications
| Color | Green |
| Content And Storage | 2 x 2.5 mL dropper bottles Store at ≤ 25°C. |
| Excitation Wavelength Range | 495/518 |
| Dye Type | Alexa Fluor™ 488 |
| For Use With (Equipment) | Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System |
| Quantity | 1 kit |
| Detection Method | Fluorescence |
| Description | ActinGreen™ 488 ReadyProbes™ Reagent (AlexaFluor™ 488 phalloidin) |
| Emission | Visible |
| Form | Liquid |
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For Research Use Only. Not for use in diagnostic procedures.
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