Invitrogen™ ReadyProbes™ F-Actin Labeling Reagents

Catalog No.	Dye Type	Excitation Wavelength Range
R37112	TRITC (Tetramethylrhodamine Isothiocyanate)	552/577
R37110	Alexa Fluor™ 488	498/520
R37178	Alexa Fluor™ 568	580/603
R37179	Alexa Fluor™ Plus 647	658/675

Catalog No. R37112 Supplier Invitrogen™ Supplier No. R37112

Description

Includes

2 x 2.5 mL dropper bottles

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 ActinGreen (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine F-actin probe (Cat. No. R415) packaged in a dropper bottle.

ActinRed 568 ReadyProbes Reagent is our high-affinity F-actin probe conjugated to our bright, red-fluorescent Alexa Fluor 568 dye. This dye is packaged in an easy to use dropper-optional bottle, allowing end-users to choose either to pipet out of the tube using the classic cap, or utilize the dropper bottle tip for typical ReadyProbes workflows.

ActinRed 647 ReadyProbes Reagent leverages our high-affinity F-actin probe conjugated to our bright Far-Red Alexa Fluor 647 Plus dye, offering enhanced brightness vs. classic Alexa Fluor 647 F-actin probe conjugates, in an easy to use format. ActinRed 647 ReadyProbes Reagent should be stored from 2-8°C for long-term, and also offers the option to leverage pipetting, if desired, via the dropper-optional cap.

These ready-to-use solutions combine superior brightness with exceptional F-actin staining. ReadyProbes actin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Specifications

• Color: Red-Orange
• Content And Storage: •2 x 2.5 mL dropper bottles
  •Store at 15°C to 30°C
  •Protect from light
  •Do not freeze
• Excitation Wavelength Range: 552/577
• Dye Type: TRITC (Tetramethylrhodamine Isothiocyanate)
• Format: Dropper bottles
• For Use With (Application): Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy
• For Use With (Equipment): Confocal Microscope, EVOS™, CellInsight™ Cell Imaging Instruments, Fluorescence Microscope, Flow Cytometer
• No. of Tests: 75 Tests
• Reactivity: F-actin
• Quantity: 2 dropper bottle(s) kit
• Detection Method: Fluorescence
• Delivery Type: Add 2 drops (80 μL) per 1 mL of medium
• Description: ActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)
• Emission: 577 nm
• Form: Ready-to-use aqueous solution
• Product Line: ActinRed, ReadyProbes
• Target: F-actin Cytoskeleton
• Technique: Cell Paint Assay
• Label Type: Fluorescent Dye
• Product Type: Cell Labeling
• Sub Cellular Localization: Cytoskeleton
• Cell Permeability: Fixed Cells
• Excitation: 552 nm

Frequently Asked Questions (FAQs)

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.