Invitrogen™ ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

Catalog No.	Color	Excitation Wavelength Range
R37178	Red	579/603 nm
R37110	Green	495/518
R37112	Red-Orange	540/565
R37179	Far Red	650 nm

Catalog No. R37178 Supplier Invitrogen™ Supplier No. R37178

Description

ActinRed™ and ActinGreen™ ReadyProbes™ reagents assist with bright, selective F-actin labeling in fixed cells and tissues. The ready-to-use dropper or dual-use bottle enables dropwise dispensing or direct pipetting for fast, flexible cytoskeleton imaging.

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 ActinGreen (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine F-actin probe (Cat. No. R415) packaged in a dropper bottle.

ActinRed 568 ReadyProbes Reagent is our high-affinity F-actin probe conjugated to our bright, red-fluorescent Alexa Fluor 568 dye. This dye is packaged in an easy to use dropper-optional bottle, allowing end-users to choose either to pipet out of the tube using the classic cap, or utilize the dropper bottle tip for typical ReadyProbes workflows.

ActinRed 647 ReadyProbes Reagent leverages our high-affinity F-actin probe conjugated to our bright Far-Red Alexa Fluor 647 Plus dye, offering enhanced brightness vs. classic Alexa Fluor 647 F-actin probe conjugates, in an easy to use format. ActinRed 647 ReadyProbes Reagent should be stored from 2-8°C for long-term, and also offers the option to leverage pipetting, if desired, via the dropper-optional cap.

These ready-to-use solutions combine superior brightness with exceptional F-actin staining. ReadyProbes actin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Specifications

• Color: Red
• Concentration: Ready-to-use
• Content And Storage: •1 Dual-use Bottle
  •Store at 15°C to 30°C
  •Protect from light
  •Do not freeze
• Excitation Wavelength Range: 579/603 nm
• Dye Type: Organic Fluorescent Dye
• Format: Dual-use Dropper Bottle
• For Use With (Application): Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy
• For Use With (Equipment): Fluorescence Microscope, Spectral Imaging Instrument, Flow Cytometer, High Content Imager
• No. of Tests: 75 Tests
• Includes: Dropper Tip, Dropper Cap
• Reactivity: F-actin
• Quantity: 1 x 6 mL
• Volume (Metric): 6 mL
• Detection Method: Fluorescence Microscopy
• Delivery Type: Add 2 drops (80 μL) to cell media
• Emission: 600 nm
• Product Line: ReadyProbes
• Target: F-actin Cytoskeleton
• Technique: Cell Paint Assay
• Label Type: Fluorescent Dye
• Product Type: Actin
• Sub Cellular Localization: Cytoskeleton
• Cell Permeability: Fixed Cells
• Synonym: Phalloidin
• Excitation: 579

For Research Use Only. Not for use in diagnostic procedures.