R&D Systems™ Recombinant A. tubingensis PNGase Protein

Description

rFm PNGase F is also available!

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. 50% ribonuclease B (6 μg) is deglycosylated by 500 ng of Recombinant A.tubingensis PNGase within 120 minutes, as measured under the described conditions.

Specifications

• Accession Number: OJI88926.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 72-85 kDa, reducing conditions; M.W. (theoretical): 60 kDa
• Quantity: 100 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived a. tubingensis PNGase At protein Leu22-Ser557 (F78I, E352G, P381S, S406Q, T412S, S414F, S451T and S555T), with an N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Species: A. tubingensis
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PNGase At