Novus Biologicals™ Recombinant Bacteria Flagellin NLRC4 Mutant His Protein

Catalog No.	Quantity
NB171719	10 μg

Catalog No. NB171719 Supplier Novus Biologicals™ Supplier No. NBP318158

Description

Recombinant Protein

Detected by human & mouse TLR5. Not detected by mouse NLRC4. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1 ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.

Specifications

• Accession Number: NP_460912
• Concentration: LYOPH
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from PBS
• Quantity: 10 μg
• Storage Requirements: Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles.
• Endotoxin Concentration: <0.01 EU/μg purified protein (LAL test; Lonza).
• Reconstitution: Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)
• Research Category: Immunology, Innate Immunity
• Purity or Quality Grade: >95% pure by SDS-PAGE
• Protein: Flagellin