R&D Systems™ Recombinant Bovine VEGF 164 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured in a cell proliferation assay using bAEC bovine aortic endothelial cells. The ED50 for this effect is 0.3-1.8 ng/mL.

Specifications

• Accession Number: P15691
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): Observed M.W.: 21-26kDa, reducing conditions, Theoretical M.W.: 19kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived bovine VEGF protein Ala27-Arg190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Common Name: VEGF
• Gene Symbol: VEGFA
• Biological Activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is typically 0.3-1.8ng/mL.
• Conjugate: Unconjugated
• Species: Bovine
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Ala27-Arg190
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining