R&D Systems™ Recombinant C. difficile Toxin A/TcdA Protein

Catalog No.	Quantity
8619GT020	20 μg

Catalog No. 8619GT020 Supplier R&D Systems™ Supplier No. 8619GT020

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to hydrolyze UDP-Glucose. The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P25738 (msyB) & P16154 (TcdA)
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Molecular Weight (g/mol): M.W. (Observed): 78 kDa, reducing conditions; M.W. (theoretical): 79 kDa
• Quantity: 20 μg
• Source: E. coli-derived c. difficile Toxin A/TcdA protein msyB (Thr2-Arg124) (N-terminus) Accession # P25738 GSENLYFQGH C. difficile TcdA (Met1-Leu542, Arg184Asn) (N-terminus) Accession # P16154 HHHHHH (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: tcdA, ToxA, Toxin A
• Common Name: Clostridium Difficile Toxin A
• Gene Symbol: tcdA
• Biological Activity: Measured by its ability to hydrolyze UDP-Glucose. The specific activity is >15 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: C. difficile
• Recombinant: Recombinant
• Expression System: E. coli-derived msyB (Thr2-Arg124) Accession # P25738, N-terminus; GSENLYFQGH; C. difficile TcdA (Met1-Leu542, Arg184Asn) Accession # P16154; HHHHHH, C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Clostridium Difficile Toxin A